A few experimental probes have been marshaled with NagA to unveil the supply of the rate limitation for this enzyme. The primary of those experiments compared the premiums of substrate hydrolysis in H2O and D2O. Using Zn-NagA with N
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you can find tyrosine (Y223) and lysine (K139) residues that may also be contributing to the conversation Along with the phosphate moiety on the substrate. Deprotonation of either of such residues at substantial pH could lead to a fall in the value of k
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-trifluoroacetyl substituted substrate is hydrolyzed 26 periods a lot quicker in comparison login naga26 to the pure substrate nevertheless the N
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